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Author Bhargava, Kanika
Title Characterization of antimicrobial-resistant staphylococcus spp. in food animals and retail meat [electronic resource] / by Kanika Bhargava.
Publication Info. 2013
Location Call No. Status Notes
 Libraries Electronic Books  Electronic Resource - WSU ETD    AVAIL. ONLINE
Description 135 p. : ill.
Note Advisor: Yifan Zhang.
Thesis Thesis (Ph.D.) -- Wayne State University, 2013.
Summary Antimicrobial resistance in <italic>Staphylococcus</italic> spp. is a worldwide epidemic concern in hospital and community settings. Food animals and retail meat are important reservoirs of these pathogens that can pose potential threat to humans. In this dissertation, we aimed to investigate the molecular epidemiology of methicillin-resistant <italic>Staphylococcus aureus</italic> (MRSA) and methicillin-resistant Coagulase-negative Staphylococci (MRCoNS) in food animals and retail meat to provide insight into the role of agricultural environment in transmitting bacteria of human clinical significance. Furthermore, the potential application of phytochemicals as antimicrobials and antimicrobial adjuvants to control MRSA infections was explored. CoNS recovered from food animals were characterized by antimicrobial susceptibility testing, resistance gene identification and conjugation. <italic>Staphylococcus lentus</italic>, <italic>Staphylococcus sciuri</italic>, <italic>Staphylococcus xylosus</italic> and <italic>Staphylococcus haemolyticus</italic> accounted for over 96% of the isolates. Resistance to macrolides, tetracyclines, and Q/D, in addition to ß-lactam resistance was observed with 54% isolates classified as multi-drug resistant. <italic>tet</italic>(M) was conjugatively transferable from 10 tetracycline-resistant CoNS to other commensals like <italic>Enterococcus faecalis</italic> by conjugation. <italic>S. aureus</italic> was recovered from 65 of 289 meat samples, including 6 samples carrying MRSA. All MRSA isolates were USA 300, the most common community-associated MRSA clone. They exhibited similar molecular profiles (SCC<italic>mec</italic>IV, ST8, <italic>pvl</italic> positive and <italic>agr</italic>I) by various sub typing methods, except for <italic>spa</italic> typing, which identified 2 distinct <italic>spa</italic> types, t008 and t2031.
Multiple CoNS species, including those that have been speculated as ancestral species of <italic>mec</italic>(A), such as <italic>S. sciuri</italic> and <italic>S. fleuretti</italic>, were prevalent in retail meat. From our SCC<italic>mec</italic> typing experiments, we identified SCC<italic>mec</italic> types IV and V in MRCoNS which has been previously found in MRSA from meat. Most of the phytochemical emulsions demonstrated antimicrobial activity against MRSA. Curcumin, t-cinnamaldehyde and garcinol (MIC: 4-16ug/ml, 312.5ul/L and 2ug/ml) were highly effective in inhibition, whereas, t-cinnamaldehyde and curcumin combinations showed synergistic or additive effect with commercial antimicrobials (cefoxitin, tetracycline and erythromycin) against MRSA. In conclusion, food animals and retail meat provide a diverse reservoir of antimicrobial-resistant <italic>Staphylococcus</italic> spp. Multidrug resistance is common in CoNS in animals. The presence of MRSA and MRCoNS in meats may pose potential threat of infection to individuals who handle the meat. The molecular composition of MRCoNS and MSSA strains clearly represent genetic background favorable for the emergence of MRSA. Antimicrobial nano-emulsions of phytochemicals and their combinations with commercial antimicrobials offer alternatives to control MRSA infections.
Subject Food science
Added Title Wayne State University thesis (Ph.D.) : Nutrition and Food Science
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